This study was conducted to determine if early feeding experience can affect subsequent prey choice in lynx spiderlings (Oxyopes salticus). After emergence from the egg sac, three groups of 10 spiderlings were each fed exclusively for a one-week period on one of three naturally-occurring prey species (crickets): group 1 fed on nymphs of Oecanthus californicus; group 2 (Neobarrettia spinosa); group 3 (Ceuthophilus conicaudus). Following this, they were tested for subsequent prey preference in choice tests conducted in a plastic arena. Each spiderling was presented simultaneously with one individual of each prey species in a randomized design. Spiderlings exhibited a significant first preference for the original diet. Thus, experience with certain foods encountered by newly hatched spiderlings can affect subsequent prey preference in this species.